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antibody to cd81 gtx31381  (GeneTex)

 
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    Structured Review

    GeneTex antibody to cd81 gtx31381
    Antibody To Cd81 Gtx31381, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd81+gtx31381+antibody/anti+cd81/pm38609899-101-0-7
    Average 90 stars, based on 1 article reviews
    antibody to cd81 gtx31381 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunocytochemistry:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Cell Culture:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Transmission Assay:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Electron Microscopy:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Isolation:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Western Blot:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Expressing:

    Article Title: Human Olfactory Ensheathing Cell-Derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOAB-CD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).

    Article Title: Human Olfactory Ensheathing Cell-derived Extracellular Cesicles: miRNA Profile and Neuroprotective Effect.
    Article Snippet: Primary antibodies used in this study were as follows: CD81 (1:1000; GTX31381, GeneTex, Inc.), CD9 (1:1000; GTX66709, GeneTex, Inc.), CD63 (1:1000; EXOABCD63A-1, System Biosciences, Inc.), TSG101 (1:1000; GTX70255, GeneTex, Inc.), BCl-2 (1:1000; GTX100064, GeneTex, Inc.), Bax (1:1000; GTX109683, Genetex, Inc.) caspase 3 cleaved Asp175 (1:800; GTX86952, GeneTex, Inc.), and beta-actin (1:20000; GTX109639, GeneTex, Inc.).



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    antibody to cd81 gtx31381  (GeneTex)
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    GeneTex antibody to cd81 gtx31381
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    ALPL deficiency inhibits the pro-angiogenesis ability of hBMMSCs exosomes. (A) Transmission electron microscopy exhibited the typical morphology of hBMMSCs exosomes. Scale bar, 100 nm. (B) Nanoparticle size distribution of hBMMSCs exosomes. (C) Western Blot analysis of exosomal proteins including CD63, CD9, <t>CD81</t> and TSG101. (D) The uptake of hBMMSCs exosomes by ECs. ECs were incubated with PKH26-lablled exosomes (PKH26-exo; PKH26 is shown in red). ECs were co-stained with phalloidin (green) and nuclei were stained with DAPI (blue). Scale bar 50 μm. Magnification image of the area marked by white box was shown in right panel. Scale bar 25 μm. (E) The migration rate of ECs cultured in different types of exosomes at equal concentrations (25 µg/mL), as assessed by scratch wound healing assay at 0 and 12 h. (F) The migration ability of ECs, as assessed by migration assay after 6 h of culture. (G) The tube formation capacity of ECs, as assessed by tube formation assay after 3 h of culture. ( H-J) The quantitative assay for scratch wound healing assay (H) migration assay (I) and tube formation assay (J) respectively. ECs cultured with medium was served as the control group (Ctrl). All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001
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    cd81 gtx31381 antibody  (GeneTex)
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    ALPL deficiency inhibits the pro-angiogenesis ability of hBMMSCs exosomes. (A) Transmission electron microscopy exhibited the typical morphology of hBMMSCs exosomes. Scale bar, 100 nm. (B) Nanoparticle size distribution of hBMMSCs exosomes. (C) Western Blot analysis of exosomal proteins including CD63, CD9, <t>CD81</t> and TSG101. (D) The uptake of hBMMSCs exosomes by ECs. ECs were incubated with PKH26-lablled exosomes (PKH26-exo; PKH26 is shown in red). ECs were co-stained with phalloidin (green) and nuclei were stained with DAPI (blue). Scale bar 50 μm. Magnification image of the area marked by white box was shown in right panel. Scale bar 25 μm. (E) The migration rate of ECs cultured in different types of exosomes at equal concentrations (25 µg/mL), as assessed by scratch wound healing assay at 0 and 12 h. (F) The migration ability of ECs, as assessed by migration assay after 6 h of culture. (G) The tube formation capacity of ECs, as assessed by tube formation assay after 3 h of culture. ( H-J) The quantitative assay for scratch wound healing assay (H) migration assay (I) and tube formation assay (J) respectively. ECs cultured with medium was served as the control group (Ctrl). All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001
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    Image Search Results


    ALPL deficiency inhibits the pro-angiogenesis ability of hBMMSCs exosomes. (A) Transmission electron microscopy exhibited the typical morphology of hBMMSCs exosomes. Scale bar, 100 nm. (B) Nanoparticle size distribution of hBMMSCs exosomes. (C) Western Blot analysis of exosomal proteins including CD63, CD9, CD81 and TSG101. (D) The uptake of hBMMSCs exosomes by ECs. ECs were incubated with PKH26-lablled exosomes (PKH26-exo; PKH26 is shown in red). ECs were co-stained with phalloidin (green) and nuclei were stained with DAPI (blue). Scale bar 50 μm. Magnification image of the area marked by white box was shown in right panel. Scale bar 25 μm. (E) The migration rate of ECs cultured in different types of exosomes at equal concentrations (25 µg/mL), as assessed by scratch wound healing assay at 0 and 12 h. (F) The migration ability of ECs, as assessed by migration assay after 6 h of culture. (G) The tube formation capacity of ECs, as assessed by tube formation assay after 3 h of culture. ( H-J) The quantitative assay for scratch wound healing assay (H) migration assay (I) and tube formation assay (J) respectively. ECs cultured with medium was served as the control group (Ctrl). All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion

    doi: 10.1186/s12951-024-02396-6

    Figure Lengend Snippet: ALPL deficiency inhibits the pro-angiogenesis ability of hBMMSCs exosomes. (A) Transmission electron microscopy exhibited the typical morphology of hBMMSCs exosomes. Scale bar, 100 nm. (B) Nanoparticle size distribution of hBMMSCs exosomes. (C) Western Blot analysis of exosomal proteins including CD63, CD9, CD81 and TSG101. (D) The uptake of hBMMSCs exosomes by ECs. ECs were incubated with PKH26-lablled exosomes (PKH26-exo; PKH26 is shown in red). ECs were co-stained with phalloidin (green) and nuclei were stained with DAPI (blue). Scale bar 50 μm. Magnification image of the area marked by white box was shown in right panel. Scale bar 25 μm. (E) The migration rate of ECs cultured in different types of exosomes at equal concentrations (25 µg/mL), as assessed by scratch wound healing assay at 0 and 12 h. (F) The migration ability of ECs, as assessed by migration assay after 6 h of culture. (G) The tube formation capacity of ECs, as assessed by tube formation assay after 3 h of culture. ( H-J) The quantitative assay for scratch wound healing assay (H) migration assay (I) and tube formation assay (J) respectively. ECs cultured with medium was served as the control group (Ctrl). All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Antibody to CD81 (GTX31381) was purchased from GeneTex (Texas, USA).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot, Incubation, Staining, Migration, Cell Culture, Wound Healing Assay, Tube Formation Assay, Generated, Standard Deviation

    ALPL deficiency induces excessive anti-angiogenic exosomes secretion in hBMMSCs. (A-B) Western Blot analysis showed PDGFBB, VEGF, Angiostatin and Endostatin expression in Nor and HPP exosomes. (C) ELISA analysis of PDGFBB, VEGF, Angiostatin and Endostatin levels in the Nor and HPP group. (D) Western Blot showed exosomes from Nor and HPP hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of Nor and HPP hBMMSCs were loaded for Western Blot. ( E) Exosomes volumes derived from Nor and HPP hBMMSCs were detected by exosomes ELISA complete kit. (F-G) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (H) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in Nor and HPP hBMMSCs. Scale bar, 10 μm. (I) Western Blot showed exosomes from HPP and HPP + pLenti-ALPL hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of HPP and HPP + pLenti-ALPL hBMMSCs were loaded for Western Blot. (J) Exosomes volumes derived from HPP and HPP + pLenti-ALPL hBMMSCs were detected by exosomes ELISA complete kit. (K-L) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (M) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in HPP and HPP + pLenti-ALPL hBMMSCs. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion

    doi: 10.1186/s12951-024-02396-6

    Figure Lengend Snippet: ALPL deficiency induces excessive anti-angiogenic exosomes secretion in hBMMSCs. (A-B) Western Blot analysis showed PDGFBB, VEGF, Angiostatin and Endostatin expression in Nor and HPP exosomes. (C) ELISA analysis of PDGFBB, VEGF, Angiostatin and Endostatin levels in the Nor and HPP group. (D) Western Blot showed exosomes from Nor and HPP hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of Nor and HPP hBMMSCs were loaded for Western Blot. ( E) Exosomes volumes derived from Nor and HPP hBMMSCs were detected by exosomes ELISA complete kit. (F-G) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (H) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in Nor and HPP hBMMSCs. Scale bar, 10 μm. (I) Western Blot showed exosomes from HPP and HPP + pLenti-ALPL hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of HPP and HPP + pLenti-ALPL hBMMSCs were loaded for Western Blot. (J) Exosomes volumes derived from HPP and HPP + pLenti-ALPL hBMMSCs were detected by exosomes ELISA complete kit. (K-L) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (M) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in HPP and HPP + pLenti-ALPL hBMMSCs. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Antibody to CD81 (GTX31381) was purchased from GeneTex (Texas, USA).

    Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Staining, Labeling, Generated, Standard Deviation

    ALPL mutation increases hBMMSCs exosomes secretion through ATP axis. (A) HPP hBMMSCs was treated with 2 U/mL ATP-apyrase (HPP + Apy) and the exosomes markers were analyzed. Exosomes proteins from equal volumes of culture supernatant of Nor, HPP and HPP + Apy hBMMSCs were loaded for Western Blot. (B) Exosomes volumes derived from each groups were detected by exosomes ELISA complete kit. (C-D) The intracellular expression of CD9 and CD81 were analyzed by Western Blot. (E) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in Nor, HPP and HPP + Apy hBMMSCs. Scale bar, 10 μm. (F) Nor hBMMSCs was treated with 10µmol/L ATP and the exosomes markers were analyzed. Exosomal proteins from equal volumes of culture supernatant of Nor and Nor + ATP hBMMSCs were loaded for Western Blot. (G) Exosomes volumes derived from Nor and Nor + ATP groups were detected by exosomes ELSA complete kit. (H-I) The intracellular expression of CD9 and CD81 were analyzed by Western Blot. (J) CD9 and CD81-positive labeled exosomal proteins localized in Nor and Nor + ATP hBMMSCs, as assessed by immunofluorescent staining. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion

    doi: 10.1186/s12951-024-02396-6

    Figure Lengend Snippet: ALPL mutation increases hBMMSCs exosomes secretion through ATP axis. (A) HPP hBMMSCs was treated with 2 U/mL ATP-apyrase (HPP + Apy) and the exosomes markers were analyzed. Exosomes proteins from equal volumes of culture supernatant of Nor, HPP and HPP + Apy hBMMSCs were loaded for Western Blot. (B) Exosomes volumes derived from each groups were detected by exosomes ELISA complete kit. (C-D) The intracellular expression of CD9 and CD81 were analyzed by Western Blot. (E) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in Nor, HPP and HPP + Apy hBMMSCs. Scale bar, 10 μm. (F) Nor hBMMSCs was treated with 10µmol/L ATP and the exosomes markers were analyzed. Exosomal proteins from equal volumes of culture supernatant of Nor and Nor + ATP hBMMSCs were loaded for Western Blot. (G) Exosomes volumes derived from Nor and Nor + ATP groups were detected by exosomes ELSA complete kit. (H-I) The intracellular expression of CD9 and CD81 were analyzed by Western Blot. (J) CD9 and CD81-positive labeled exosomal proteins localized in Nor and Nor + ATP hBMMSCs, as assessed by immunofluorescent staining. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Antibody to CD81 (GTX31381) was purchased from GeneTex (Texas, USA).

    Techniques: Mutagenesis, Western Blot, Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Staining, Labeling, Generated, Standard Deviation

    P2X7 serves as a key regulator of ALPL-ATP axis mediated exosomes secretion. (A, D) The expression of P2X7 in Nor and HPP hBMMSCs, as assessed by Western Blot analysis. (B, E) The expression of P2X7 was analyzed by Western Blot in Nor, HPP and HPP + Apy hBMMSCs. (C, F) The expression of P2X7 was analyzed by Western Blot in Nor and Nor + ATP hBMMSCs. (G) P2X7 siRNA-treated HPP hBMMSCs (HPP + siP2X7) was collected and exosomes marker were analyzed. Exosomal proteins from equal volumes of culture supernatant of Nor, HPP and HPP + siP2X7 hBMMSCs were loaded for Western Blot. (H-I) The intracellular expression of CD9 and CD81 were examined by Western Blot. (J) CD9 and CD81-positive labeled exosomal proteins localized in Nor, HPP and HPP + siP2X7 hBMMSCs. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion

    doi: 10.1186/s12951-024-02396-6

    Figure Lengend Snippet: P2X7 serves as a key regulator of ALPL-ATP axis mediated exosomes secretion. (A, D) The expression of P2X7 in Nor and HPP hBMMSCs, as assessed by Western Blot analysis. (B, E) The expression of P2X7 was analyzed by Western Blot in Nor, HPP and HPP + Apy hBMMSCs. (C, F) The expression of P2X7 was analyzed by Western Blot in Nor and Nor + ATP hBMMSCs. (G) P2X7 siRNA-treated HPP hBMMSCs (HPP + siP2X7) was collected and exosomes marker were analyzed. Exosomal proteins from equal volumes of culture supernatant of Nor, HPP and HPP + siP2X7 hBMMSCs were loaded for Western Blot. (H-I) The intracellular expression of CD9 and CD81 were examined by Western Blot. (J) CD9 and CD81-positive labeled exosomal proteins localized in Nor, HPP and HPP + siP2X7 hBMMSCs. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Antibody to CD81 (GTX31381) was purchased from GeneTex (Texas, USA).

    Techniques: Expressing, Western Blot, Marker, Labeling, Generated, Standard Deviation